Q SEPHAROSE FAST FLOW, 25 ML

Q Sepharose Fast Flow, 25 ml
Q Sepharose Fast Flow is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
Well-proven strong anion exchanger developed for industrial downstream processes.
Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
The industry standard for ion exchange chromatography during recent decades
High chemical stability allows for well proven CIP and sanitization protocols
The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.
As member of the BioProcess media range, Q Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.


Features:
•Well-proven strong anion exchanger developed for industrial downstream processes.
•Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
•The industry standard for ion exchange chromatography during recent decades
•High chemical stability allows for well proven CIP and sanitization protocols
•The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, Q Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Chemical Stability	Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol 1 M NaOH and acetic acid should be used only for cleaning purposes.
Complete Packsize	25 ml
Exclusion Limit [Mr] [Globular Proteins]	4 x 10⁶
Ion Exchanger Type	Strong anion exchanger
Ionic Capacity	0.18-0.24 mmol Cl-/ml medium
Ligand	Quaternary amine
Matrix	6% cross-linked agarose
Particle Size	45 µm-165 µm
pH Stability Cleaning-in-Place (CIP)	2-14
pH Stability Working Range	2-12
Pressure/Flow Specification	400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Storage Conditions	4 to 30°C, 20% Ethanol
BioProcess resin	Yes
Particle size, d50V	90 µm d50v is the median particle size of the cumulative volume distribution.
pH stability, CIP	2–14