Excella™ SYBR MasterMix IQ, 1250 x 20µl rxns (10 x

Excella SYBR Green MasterMix for iQ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR (qPCR) on Bio-Rad iCycler iQ® systems. This unique combination of proprietary buffer, stabilizers, and ExcellaStart™ Taq DNA polymerase deliver maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. ExcellaStart Taq DNA polymerase contains a proprietary mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (> 48 hours at room temperature). Activation of ExcellaStart Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200 bp is complete in less than 20s at 60°C.