FrameStar 384 well PCR plate, frosted wells, clear

Our Framestar 384 Well Plates are designed for high-throughput PCR. They are compatible with the majority of 384-well block PCR, qPCR and sequencing instruments, and the rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.

Features:

•Recommended for low volume PCR
•Rigid Polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
•Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
•Improved seal integrity due to thermal stability of frame
•Compatible with standard multichannel pipettes
•Ideal for use with robotic systems
•50 µl volume tubes
•Compatible with majority of 384-well block PCR, qPCR and sequencing instruments


Frosted Wells for ABi/Life Tech qPCR instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflection of light in fluorescent based assays used in real-time PCR (qPCR). This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABi/LifeTech cyclers. For this reason, the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variant of the FrameStar® 384 plate to offer ABi/LifeTech instrument users the time and money saving advantages of FrameStar®. And as the signal levels are directly comparable with ABi/LifeTech, there's no need for users to recalibrate their instruments.