SEPHAROSE 6B 1 L

Sepharose 6B, 1 l
Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
6% Agarose gel filtration media.
Proven base matrix for coupling affinity ligands
Sepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight
Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents


Features:
•6% Agarose gel filtration media.
•Proven base matrix for coupling affinity ligands

Sepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight

Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents

Autoclavable	Autoclavable, 20 min at 120°C in pH 7
Complete Packsize	1 l
Exclusion Limit	> 4 x 10⁶
Flow Velocity	<14 cm/h
Fractionation [Mr] Globular Proteins	1 x 10⁴-4 x 10⁶
Matrix	6% agarose
Particle Size	45 µm-165 µm
pH Stability Cleaning-in-Place (CIP)	4–9
pH Stability Working Range	4–9
Pressure/Flow Specification	100-200 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.
Storage Conditions	4 to 30°C, 20% Ethanol
pH stability, CIP	4–9