SP SEPHAROSE HIGH PERF. 75 ML

SP Sepharose High Performance, 75 ml
SP Sepharose High Performance is a strong cation exchange BioProcess medium with high resolution designed for intermediate and polishing steps in downstream processing
Strong cation exchanger for intermediate and final polishing steps
High resolution, high capacity separation with high recovery
High chemical stability for effective CIP/sanitization
BioProcess medium supported for industrial applications and well-established in approved processes
SP Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. SP Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.
SP Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, SP Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.


Features:
•Strong cation exchanger for intermediate and final polishing steps
•High resolution, high capacity separation with high recovery
•High chemical stability for effective CIP/sanitization
•BioProcess medium supported for industrial applications and well-established in approved processes

SP Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. SP Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.

SP Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, SP Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.

Charged Groups	- SO₃-
Chemical Stability	Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS 1 M NaOH and acetic acid should be used only for cleaning purposes.
Complete Packsize	75 ml
Exclusion Limit [Mr] [Globular Proteins]	4 x 10⁶
Ion Exchanger Type	Strong cation exchanger
Ionic Capacity	0.15-0.20 mmol H+/ml
Ligand	Sulphopropyl
Matrix	6% cross-linked agarose
Particle Size	24 µm-44 µm
pH Stability Cleaning-in-Place (CIP)	3–14
pH Stability Working Range	4–13
Pressure/Flow Specification	150 cm/h (at < 3 bar, 25°C, 10 cm bed height)
Storage Conditions	4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
BioProcess resin	Yes
Particle size, d50V	34 µm d50v is the median particle size of the cumulative volume distribution.
pH stability, CIP	3–14