NI SEPHAROSE 6FF, 25 ML

Ni Sepharose 6 Fast Flow, 25 ml
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
Designed for scaling up purification of histidine-tagged proteins
High protein binding capacity and minimal leakage of Ni₂₊ ions
As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
Available in convenient prepacked HisPrep and HisTrap column formats.
Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.
Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni₂₊ ions for purification of histidine tagged proteins.
The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni₂₊ leakage while providing high capacity.
Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support


Features:
•Designed for scaling up purification of histidine-tagged proteins
•High protein binding capacity and minimal leakage of Ni₂₊ ions
•As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
•Available in convenient prepacked HisPrep and HisTrap column formats.

Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni₂₊ ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni₂₊ leakage while providing high capacity.

Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Binding Capacity/ml Chromatography Medium	Approx. 40 mg histidine-tagged protein
Chemical Stability	Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea Ni²⁺-stripped medium.
Complete Packsize	25 ml
Matrix	6% cross-linked agarose
Metal Ion Capacity	~15 µmol Ni₂₊/ml medium
Particle Size	45 µm-165 µm
pH Stability Cleaning-in-Place (CIP)	2–14 Ni²?-stripped medium.
pH Stability Working Range	3–12 Ni²?-stripped medium.
Pressure/Flow Specification	Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Storage Conditions	4 to 30°C, 20% Ethanol
BioProcess resin	Yes
Particle size, d50V	90 µm d50v is the median particle size of the cumulative volume distribution.
pH stability, CIP	2–14 Ni²?-stripped medium.