Matrigel Matrix for Organoid Culture Phennol-Red Free, 10mL

Corning® Matrigel® Matrix for Organoid Culture, Phenol Red-free, LDEV-free, 10 mL

Corning Matrigel Matrix for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture. Verified to support both mouse and human organoid growth and differentiation from healthy and/or diseased tissue, each lot has a specific elastic modulus value indicating the stiffness of the gel formed and is qualified to form stable 3D domes commonly used in organoid culture protocols. Available in 10mL LDEV-free, Phenol Red-free format.

Corning Matrigel Matrix for Organoid culture is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Tested for ability to form and maintain stable 3D domes/droplets on multi-well plates.
• Elastic Modulus (matrix stiffness) measured using a rheometer.
• Protein concentrations determined by Lowry method
• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

Specs:
Application: Organoid Culture
Cell Types: Organoid, Organoid Lines from Primary cells and Stem cells, Stem and progenitor cells
Handling: Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.
Protein Concentration: Lot-dependent
Size: 10 mL
Source: Engelbreth-Holm-Swarm lathrytic mouse tumor
Storage Recommendation: Store at -20°C.
Surface Category: Extracellular Matrices (ECMs) and Biological Coatings
Surface Type: ECM
Usage: Vial