The fluorescent dye calcein acetoxymethylester (calcein AM) can be used with Corning FluoroBlok Cell Permeable Supports and Insert Systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays.
The cell viability indicator calcein AM is a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein. Corning Calcein AM Fluorescent Dye can be used to fluorescently pre- and post-label viable cells to perform kinetic and endpoint experiments, respectively. Note: cells are known to tolerate different fluorescent dyes for varying amounts of time in culture. While cells labeled with calcein AM should be exposed to the dye for less than eight hours, Corning DiIC12(3) can be used for several days in culture without adversely affecting cells.
Preparation and Storage
Product is shipped on dry ice. Upon receipt, store immediately at -20°C. Stable for at least three months from date of shipment.